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Promega go scripttm reverse transcriptase kit
Go Scripttm Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+scripttm+reverse+transcriptase+kit/goscripttm+reverse+transcription+system/pm37055986-132-12-17
Average 90 stars, based on 1 article reviews
go scripttm reverse transcriptase kit - by Bioz Stars, 2026-10
90/100 stars

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Reverse Transcription:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Contrasting Cd accumulation of Arabidopsis halleri populations: a role for (1→4)-β-galactan in pectin.
Article Snippet: RNA was quantified with the Nano Drop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Loughborough, UK). .. 1 μg of total RNA was used for cDNA synthesis using the Go ScriptTM Reverse Transcriptase kit (Promega). .. The cDNA was used for qRT-PCR using the Go Taq® qPCR and RT-qPCR Systems kit (Promega).

Random Hexamer:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Amplification:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Polymerase Chain Reaction:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Expressing:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

SYBR Green Assay:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Real-time Polymerase Chain Reaction:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

Quantitative RT-PCR:

Article Title: Developmental changes of rRNA ribose methylations in the mouse
Article Snippet: Primer sequences can be found in Table S2. cDNAs were fractionated by electrophoresis on a 8% polyacrylamide/7 M urea denaturing gel, the gel was dried and radioactive signals from the probes were revealed using a TyphoonTM Biomolecular Imager (Amersham). .. Analysis of Gas5 expression by RT-qPCR One microgram of whole-cell RNA was reverse transcribed with random hexamer primers using Go ScriptTM Reverse Transcriptase kit (Promega) at 42°C for 60 min. 1/20 of the RT reaction was amplified by PCR using GoTaq polymerase (Promega). mRNA expression was performed using the IQTM Custom SYBR® Green Supermix (Bio-Rad) qPCR on a CFX96 TM Bio-Rad Real-Time Thermal Cycler. ..

cDNA Synthesis:

Article Title: Contrasting Cd accumulation of Arabidopsis halleri populations: a role for (1→4)-β-galactan in pectin.
Article Snippet: RNA was quantified with the Nano Drop 2000 UV-Vis Spectrophotometer (Thermo Scientific, Loughborough, UK). .. 1 μg of total RNA was used for cDNA synthesis using the Go ScriptTM Reverse Transcriptase kit (Promega). .. The cDNA was used for qRT-PCR using the Go Taq® qPCR and RT-qPCR Systems kit (Promega).



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